Trump was due on Monday to sign a memorandum directing US Trade Representative Robert Lighthizer to determine whether any Chinese laws, policies or practices discriminate against or harm American innovators and technology companies.
The investigation could lead to sanctions against Beijing.
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"Fighting a trade war has no future. There will be no winner and everybody will lose," Hua said at a regular news briefing.
"I believe China and the US should continue to work together for the stable and sound development of China-US economic and trade relations," she said.
US officials, who spoke on condition of anonymity, bluntly accused China on Saturday of "stealing our intellectual property" — long a concern of Western companies seeking a share of the enormous Chinese market.
The looming investigation follows high tensions between Washington and Beijing. Trump has accused China of failing to rein in the nuclear ambitions of its ally North Korea.
The US officials, however, said the North Korean issue and the investigation into China's trade practices are not linked.
China on Monday announced it would ban from Tuesday imports of iron, iron ore and seafood from North Korea as it follows through on new UN sanctions approved earlier this month.
"I want to say that China-US cooperation should be based on mutual respect and mutual trust," Hua said.
"The Korean peninsula nuclear issue and the China-US trade issue are totally different and it's not appropriate to use one issue as a tool to keep pressure on the other issue."
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