The layoffs, which come six weeks after top boss Elon Musk reportedly told staff that there would not be further retrenchment, could reduce the company's headcount to under 2,000, according to the report.
Twitter did not immediately respond to a Reuters request for comment.
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Musk had said in November that Twitter was facing "a massive drop in revenue" as advertisers dropped out.
Twitter's revenue for the fourth quarter fell about 35 per cent to $1.025 billion, a top ad executive revealed at a staff meeting, online publication the Information reported on Wednesday.
Staff cuts so far, which also included employees working in the content moderation division, have stoked fears of a surge in hate speech on the platform.
(Reporting by Yuvraj Malik in Bengaluru; Editing by Maju Samuel)
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