In Madhya Pradesh, eight birds have died in Gwalior zoo in the last 48 hours from infection, officials' state.
Samples have been sent to veterinary hospital for investigation and Precautionary measures have been taken at the zoo in the wake of reports of bird flu from some areas of the country.
Meanwhile, National Zoological Park in Delhi, which has been closed temporarily, has reported fresh cases of deaths of pelican birds.
The zoo was shut down on Wednesday after nine birds, including painted storks, ducks and pelicans died of Avian influenza at its premises between October 14 and October 17.
Meanwhile, a team of Central Zoo Authority is currently inspecting all the enclosures at the zoo.
Also, the Delhi government has shut down the Deer Park in Hauz Khas after it suspected that two birds found dead inside the park could be infected with bird flu.
Around 100 more samples have been collected in the last two days from Ghazipur, Okhla Bird Sanctuary, Najafgarh drain, Yamuna Biodiversity Park and the Central Park in Hauz Khas and sent to a specialised Regional Disease Diagnostic Laboratory in Jalandhar.