The formidable pair will add excitement to the event that has an impressive line-up of 4 singles players from the top 20.
Bopanna's former partner Mahesh Bhupathi would be teaming up with World No.5 doubles player Canada's Daniel Nestor.
Bhupathi and Bopanna decided to end their partnership after the season-ending season-ending London Masters. It was a good year for them during which they won two titles at Dubai and Paris and a runner-up finish at the London Masters.
Boppana and Ram had earlier appeared as a team at the Australian Open in 2008, where they made the pre-quarters.
Karti Chidambaram, chairman of the organising committee, said: "We are thrilled to welcome Rohan Bopanna and Rajeev Ram as they begin their journey at the Aircel Chennai Open 2013. Both players are immensely talented and will definitely be a tough pair to defeat. We wish them the best in their new endeavour."