The decision was taken at a meeting chaired by top party office-bearers O Panneerselvam and K Palaniswami, AIADMK sources said.
The party decided to remove, among others, key Dhinakaran aides P Vetrivel and Thangatamilselvan, AIADMK district secretary in Chennai and Theni respectively, the sources said.
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Though AIADMK supporters are divided between Dhinakaran and the Palaniswami-led camps, many office-bearers from the rival group hold party posts.
In a jolt to the ruling dispensation, Dhinakaran defeated its candidate E Madhusudhanan by 40,707 votes in the December 21 RK Nagar bypoll.
The votes were counted yesterday.