"Hemlal Murmu has resigned from Assembly membership," Speaker Shashank Sekhar Bhokta said here.
"Bidyut Baran Mahto also called me over the phone and said he is sending his resignation letter today," he added.
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Both Murmu and Mahto, who won Assembly elections on JMM ticket, joined the BJP after being in the party for several years.
Yesterday Chandrasekhar Dubey, who quit Congress and joined the Trinamool Congress, had resigned as MLA.
After Mahto submits his resignation, the strength of the 82-member Jharkhand Assembly would be reduced to 79.