Delhi resident Dinesh Mahajan, along with four members of his family, was going to Mathura in his car, police said.
The car's tyre burst on the expressway and Mahajan lost control over the vehicle leading to which it overturned along Agra Greater Noida road, police said.
All the injured were rushed to a hospital, where Dinesh's mother Tapaswani Jamuna Bai (60) was declared brought dead by doctors.
The condition of the injured is stated to be stable.