The 1965 model equipped with revolving number plates, hidden guns and hubcap blades is one of four made for the franchise, reported Sun online.
It never appeared on-screen but was used to promote Bond's fourth outing, 'Thunderball'.
Bond first drove a DB5 in 'Goldfinger' starring Sean Connery.
Surrey-based sellers, Aston Martin specialists RS Williams, described the 150mph DB5 as the "most famous car in the world."