Two pedestrians also sustained serious injuries in the accident that took place last evening, the police said.
The accident occurred when a truck hit a two-wheeler near Srirampur on Paradeep-Daitary Express Highway, killing the motorcyclist and the pillion rider.
The victims have been identified as Dillip Mallik (50) and his nephew Saisankar Mallik (14) of Nuagaon village in Jajpur district, they said.
The injured were identified as Kumudini Mallik (36) and her 10 year-old-son Pupun. They were admitted to the SCB Medical College and Hospital in Cuttack.
The blockade was withdrawn after officials reached the spot and assured them of legal action against unauthorised overloaded vehicles.