Documents filed in the case of Sheheryar and Raaes Qazi show the alleged attack occurred on April 8. US District Judge Robert Scola issued an order yesterday setting a May 9 hearing that says the incident happened at a secure facility where defendants and their lawyers can examine classified evidence.
"This incident is currently under investigation and may lead to the filing of additional charges," prosecutors said in a recent court filing.
"Any time you have a physical altercation, it's serious," he added.
The two men are already accused of several terrorism-related charges stemming from an alleged plot to blow up New York landmarks to avenge overseas deaths in US drone attacks. Both brothers have pleaded not guilty.
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Investigators found bomb-making materials and components at the Qazi family home and Raees Qazi, 21, traveled to New York in late 2012 in hopes of finding a job and carrying out his plot, prosecutors have said. But he was unsuccessful and eventually returned to Florida on a Greyhound bus.
Scola said in light of the assault he wants the hearing next week to also focus on whether additional security is needed in his courtroom for the Qazi case or in the classified documents viewing location, known as the Secure Compartmentalized Information Facility or SCIF.
Trial for the two brothers is currently set for November. The case is also notable because of a legal battle over whether prosecutors must disclose if evidence from once-secret US surveillance programmes will be used against the defendants.