The easternmost district of Ak-Suu in the Central Asian country was on lockdown while police guarded the hospitals where 15-year-old Temirbek Isakunov was treated and died last Thursday.
The emergency ministry said that three more people from the same village as the victim were hospitalised today on suspicion of being infected with the deadly disease.
"Residents of Sary-Kamysh... Came to the hospital at 12:30 am," the ministry said in a statement. "They are now under medical care."
"They had contact with the deceased 15-year-old," the source said.
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The health ministry however denied that any new patients have been infected.
Temirbek, a herdboy from the village of Ichke-Zhergez, is thought to have become infected after being bitten by an oriental flea carried by a marmot that he reportedly prepared for food.
Officials had isolated 131 people as of today to prevent the possible spread of the disease, said Asylbek Sydykanov, the ministry's public health official.
Bubonic plague is a bacterial infection that is a strain of the "Black Death", a virulent disease that killed tens of millions of people in 14th-century Europe. Primarily an animal disease, it is extremely rare in humans.