It was the largest airlift of lions in history, said Jan Creamer, president of Animal Defenders International, which carried out the operation yesterday.
"These lion have suffered tremendously," Creamer said as the lions in crates were loaded onto trucks.
"They lived in small cages on the backs of trucks for their entire lives. Some of them had their teeth bashed in with steel pipes in circuses in Colombia and Peru. Some of them had their claws removed ... It is a wonderful feeling to bring them back to their home."
The lions will be placed in quarantine in enclosures at the 5,000 hectare Emoya Big Cat Sanctuary in Vaalwater in northern South Africa, started three years ago by a single mother and her teenage daughter.
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The 33 lions will be monitored by a vet for their first weeks in Africa. They will then be introduced to each other in a one hectare bonding enclosure. Many of the lions were never allowed to have direct physical contact with other lions and have never been together without a fence or a cage separating them.
The enclosures will be fitted with drinking pools, platforms and toys to ensure the lions don't become bored and will be steadily expanded as they become familiar with their new life, Heuser said.
Emoya, in an area with a mix of habitats including mountainous regions, rolling grasslands, forests, cliff caves and river gorges, has a strict non-breeding policy, Heuser told The Associated Press.
"The animals have no conservation value whatsoever. Many of them have been inbred," she said. "When we are sure that no breeding will take place, we allow males to interact with females. By then a pattern will have emerged ... And we will know which lions can be placed together.