The incident took place near Kasara when the truck carrying around 30 people hit a wall and fell off the culvert, they said.
The passengers were on way to Nashik to attend a marriage function.
The four deceased have been identified as Shobha Madhukar Dhonde, Atul Chandrashekar More, Lilabhai Pandurang Bhole and a 17-year-old boy Akash Prakash Bhole, police said.
Nineteen people, including 13 women, who were injured in the accident were being treated at Nashik Civil Hospital.