Maitree Express has hit a car on a level-crossing in Kaliakoir upazila of Gazipur around 10:15 am, Motaleb Mia, officer-in-charge (OC) of Kaliakoir Police Station, said.
A compartment of the train was derailed one kilometre away from the accident spot, a security officer of the train service said.
Another report quoted witnesses as saying that the Kolkata-bound train hit the car, sending it on a roll.
Five members of a family - two women, two children and a man - were killed in the mishap, that also halted train communication between Dhaka and the northern parts of the country.
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