The first night went without a hitch.
The beginning of the overnight subway services has put the British capital in league with other major cities around the world offering a 24-hour underground transport network.
The service is likely to come as a boost for revellers, tourists and shift workers who on weekend nights will be able to travel on the network at any chosen hour.
"What's important is we got the detail and the planning right. I'm really pleased that 100 days or so after becoming the mayor we've got that right," he said as he took a ride on the first train on the Victoria Line Underground last night.
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The plan to run services beyond the midnight deadline was announced in November 2013 and was to kick-off in September last year. After a number of missed start dates, the first night Tubes - finally started this weekend.
TfL said 50,000 people used the Night Tube last night. The numbers are expected to reach 200,000 once all five lines are open, BBC reported.
Night Tube services are expected to add 6.4 billion pounds to London's economy by 2030, creating 500,000 jobs, it said.
The plan was first announced by former London mayor Boris Johnson but faced delays as workers' unions staged a series of strikes during July and August last year before anagreement was reached on pay in March this year.
London Underground appointed Mark Wild asmanaging directorto launch the services considering his experience of launching a similar service in Melbourne, Australia, earlier this year.
"What happened there is what I expect will happen here. It's not just for revellers. It's for the night-time economy: people working in hospitality, shift workers, nurses. We expect a nice mix of people who enjoy the fantastic nightlife of the city, but it's also a lot about giving people mobility," Wild said.
Around 100 BritishTransport police officers will be on patrol across the network on Friday and Saturday nights.
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