Chile's National Seismological Center said the latest quake struck at 3:27 PM (1827 GMT), with its epicenter in the town of Cobquecura, about 500 kilometers (300 miles) south of the capital Santiago.
The National Emergency Office said in an initial report that there were no casualties or major damage and the quake did not create a tsunami risk.
Chilean TV showed people evacuated from buildings in Concepcion and reported some problems with phone lines.
It killed more than 500 people and inflicted an estimated USD 30 billion in damages.