The finding places the cactus among the most threatened plant groups on Earth, ahead of mammals and birds and just behind corals, according to the IUCN, an inter-governmental conservation organization.
"The results of this assessment come as a shock to us," lead researcher Barbara Goettsch, co-chair of the IUCN's Cactus and Succulent Plant Specialist Group, said in a statement.
"We did not expect cacti to be so highly threatened."
The IUCN (International Union for the Conservation of Nature) Red List is widely recognised as the gold standard for measuring extinction risk for animals and plants.
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They are an essential sources of sustenance and water for deer, woodrats, rabbits, coyotes, lizards and tortoises which, in return, help spread cacti seeds.
Cactus flowers supply nectar to hummingbirds and bats, along with bees, moths and other insects that help pollinate the largely desert-dwelling plants.
In many parts of Central and South America, cacti are used by people for food and medicine.
Mexico's prickly pear cactus stem, for example, is highly nutritious, while the root of another species listed as near threatened -- Ariocarpus kotschoubeyanus -- is commonly used as an anti-inflammatory.
The top threat to cacti is expanding agriculture, especially in northern Mexico and the southern part of South America.
Species native to coastal areas are being decimated by residential and commercial development, while in southern Brazil conversion of land for eucalyptus plantations is harming at least 27 species, some of them already on the endangered list.
"Their loss could have far-reaching consequences for the biodiversity and ecology of arid lands and for local communities dependent on wild-harvested fruits and stems," Goettsch said.