The couple moved the apex court challenging the CBI court's order rejecting their plea that 14 other witnesses, including ADG (law and order) and the then CBI Joint Director Arun Kumar, also be called to record statements.
The trial court had yesterday rejected the defence plea and ordered to record the statements of the Talwar couple, who are the main accused.
The CBI had told the court that 14-year-old Aarushi's murder five years back was carried out by her parents Rajesh and Nupur and no outsider was present.
In his deposition before the court, Kaul, who headed the CBI probe, had told the court that their investigations revealed no evidence of any third person's entry in the house.
Aarushi was found dead with her throat slit in her bedroom on May 16, 2008.
The initial suspicion went on Hemraj whose body was later found in the terrace at their residence in Jalvayu Vihar in Noida on the outskirts of Delhi.