Following on from losses to the tournament's host in the first two games of the three-match series, South Africa's bowlers caused an English collapse as Eoin Morgan's men were reduced to 20 for 6 after just five overs, before being bowled out for 153.
The seven-wicket win marked a memorable end to the series for both sides - for South Africa, a sigh of relief, while England was humbled and reminded of the challenge it faces if it is to succeed on home soil.
And while de Villiers admits series defeat to England was not how South Africa would have liked to have started its Champions Trophy campaign, he insists it will do little to knock its focus from the task at hand.
"I was pretty happy with our preparations for the Champions Trophy, although obviously you don't want to lose a series that you play in," said de Villiers, who will enter the tournament ranked the world's best batsman.
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"We're still ranked the No.1 team in the world going into the tournament, so there are quite a few reasons for us to be confident, but we're still humbled at the same time and know that we have to work hard and take it one step at a time to win this.
"Anything can happen on the day in one-day cricket, that's a fact, and especially with a tournament like the ICC Champions Trophy.
South Africa starts its Champions Trophy campaign against Sri Lanka at The Oval on Saturday, as it chases its first title since the tournament's inaugural year in 1998, in what was then called the ICC Knockout Trophy.
And de Villiers is under no illusions as to the importance of a winning start to proceedings, emphasising the need for his men to focus on solely the next game on the calendar.