The remarks follow Netanyahu's claim during an address rejecting criticism of Israeli settlement building that the Palestinians were seeking a state with "no Jews".
Netanyahu called that "ethnic cleansing," drawing a strong rebuke from the United States.
Speaking yesterday night before the Eid al-Adha Muslim festival, Abbas said the Israelis "don't want to progress one iota towards peace, in spite of their deceptive allegations."
"On the contrary, they go deeper with their settlements, infringement of holy places, ethnic cleansing and deliberate killing," he said, according to official Palestinian news agency WAFA.
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"There's a phrase for that, it is called ethnic cleansing," he said.
The US State Department called the video "unhelpful" and "inappropriate".
"We obviously strongly disagree with the characterisation that those who oppose settlement activity or view it as an obstacle to peace are somehow calling for ethnic cleansing of Jews from the West Bank," spokeswoman Elizabeth Trudeau said.
The Palestinian leader has made the allegation against Israel before.
Palestinians accuse Israel of ethnic cleansing during the war surrounding the creation of the Jewish state in 1948, a claim Israel denies.
Netanyahu has said he is ready to meet Abbas at any time, but without preconditions.
Abbas did not say whether he had preconditions for such a meeting, but in the past the Palestinians have demanded a freeze in Israeli settlement building, a deadline for the end of the occupation of the West Bank and the release of prisoners.
"In recent days, we have agreed to a meeting with Israeli Prime Minister Benjamin Netanyahu on the kind invitation of the Russian president," Abbas said.
Peace efforts have been at a standstill since a US-led initiative collapsed in April 2014.