The 'Coalition for a GM-Free India', a platform of hundreds of organisations representing farmers, consumers, and scientists, wrote to Union Environment Minister Anil Dave expressing "disappointment and shock" over the approval by the Genetic Engineering Appraisal Committee (GEAC).
They demanded the regulatory body be immediately dissolved given its "repeated unaccountable functioning".
Meanwhile, Environment activists Vandana Shiva asserted that GM mustard will lead to "genetic contamination" of pure organic mustard and pollution of food.
Environment Minister Anil Madhav Dave now has to take the final call on approval of GM Mustard.
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Ministry sources told PTI that although they were expecting the approval from the Minister today itself, the process has been delayed by a week.
"By clearing GM mustard, the GEAC has shown itself to be anti-science, anti-farmers, anti-environment and anti- consumers.
"We appeal to you (Dave) to summarily reject the application for approval of GM-Mustard. We also urge you to dissolve the GEAC immediately, given their repeated unaccountable functioning," the Coalition said in the letter written to Dave.
With its green signal, the GEAC has pushed the hybrid plant developed by scientists at the Delhi University closer to becoming the country's first edible GM crop.
"The GEAC is a compromised body, which works only for corporate interests. Every word of their safety dossier was prepared by corporations.
The Coalition also said the GM HT Mustard is a "hazardous" herbicide-tolerant food crop, which has adverse impact on a large number of Indian farmers, agricultural workers and consumers.
It said that previously, it has shown, through rigorous analysis of all available materials that how it will increase chemicals in food and farms and how regulators should have never allowed it to proceed this far.
"The GEAC has ignored all the many valid questions raised by scientists and others and chose to function in an unscientific and biased fashion. Importantly, there was no integrity apparent in the processes adopted," the Coalition said in a statement.
It said when the rest of the world is shunning GM crops, India would be "foolish" to rush into GM mustard commercialisation.
"There is absolutely no reason, not a single one, why the GEAC should have recommended this for approval. Herbicide Tolerant crops increase chemical usage.