He joins Barclays chief executive officer Bob Diamond and chairman Marcus Agius who have already resigned.
The top management has come under intense pressure, including political, after the Barclays was slapped with 290 million pound (USD 451 million) fine by the US and the UK authorities to settle the charges of manipulating global benchmark lending rates.
In a statement today, Barclays said Missier has resigned from the company.
"My 15 years at Barclays have been a time of great accomplishment, both for me personally and for the bank," Missier said.
Earlier in the day, the resignation of Diamond -- who has served as the company for 16 years -- was announced by Barclays.
"...The external pressure placed on Barclays has reached a level that risks damaging the franchise