The charter flight AI 3707 will depart from Kolkata at 2100 hours and arrive at Port Blair at 2300 hours.
The return flight AI3708 will leave Port Blair at 2340 hours and land back in Kolkata at 0125 hours, Air India said today.
The Wadia group-promoted GoAir currently operates night flights to Port Blair's Veer Savarkar International Airport from Bengaluru.
"Air India, in fact, had pioneered night landing operations in Port Blair, by carrying out safety risks assessment through a test flight to check the viability of the night operations to Port Blair," the Government-run carrier said.