Segarhad held the Nellithope segment for two consecutive terms in 2006 and 2011.
However, he was defeated by A John Kumar of Congress in the assembly elections held in May this year.
Segar filed his papers before the Returning Officer G Malarkannan.
The vacancy in Nellithope arose after John Kumar quit in September 15 this year to clear the decks for Chief Minister V Narayanasamy, who is not a member of the assembly, to contest.