According to chief medical officer of animal husbandry department Bhupender Singh, the four horses were put down by poisonous injection as a precaution after lab tests confirmed presence of the disease in the animals in Dera Lalsingh village in Shamli district.
The bodies were buried after postmortem.
The department has sanctioned Rs 16,000 per horse to the owner of the dead horses as compensation.
Singh said glanders can be contracted by humans as well.