Bayliss said all-rounder Ali (side strain) and fast bowler Finn (knee), who also missed England's drawn opening tour game in Perth, will sit out the four-dayer under lights against a Cricket Australia XI, starting on Wednesday.
"Finn is still not right, we won't know about him for another couple of days yet," Bayliss told reporters here today.
"Mo Ali is more precautionary now, we will just leave him out of this one and make sure he's right."
"We have probably got a batting spot to finalise and probably a fourth pace position so there's a little bit of competition from within which is good," Bayliss said.
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England have one further four-day warm-up game in Townsville from November 15 before the first Test.
Bayliss said the team was focused on becoming familiar with the day-night conditions and playing with a pink ball ahead of the second Test at Adelaide Oval.
England have only played one day-night Test, which they lost to the West Indies last August.
"If the ball moves around a little bit more, that is what we like to see more of.
"Our batters are more used to playing on wickets that do a bit more sideways and obviously the bowlers bowling on it as well, moreso than the Australian guys."
Former Test fast bowler Ryan Harris, coach of the CA XI team, said he wants to send the tourists to Brisbane low on confidence and with as little insight as possible into batting against the pink ball.
Harris said he wants to reduce batting opportunities for England during the twilight period that has proven prohibitive for batsmen.
"We are not here to make it easy for England," Harris said.
"When it gets to dusk the grass almost seems to sit up and it zips around. It can be tough to bat around that time.
"That is where we will make it as hard as we can for England. Hopefully they are not batting around that time, that is my plan.