The revenue of 32.15 billion yuan (USD 4.85 billion) topped market estimates, pushing Alibaba's share price up by 5 per cent to about USD 92 in New York.
Gross Merchandise Volume (GMV) transacted in Alibaba's China retail marketplace was 837 billion yuan (USD 125 billion), up by 24 per cent year on year, state-run Xinhua news agency reported.
The number of annual active buyers on China retail marketplace rose to 434 million by the end of the first fiscal quarter.
The revenue of cloud computing rose by 156 per cent, primarily driven by an increase in the number of paying customers.
The revenue from digital media and entertainment business in the quarter was over 3.13 billion yuan, an increase of 286 per cent from 821 million yuan in the same period of 2015.
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