Knox was sentenced to 28 1/2 years in prison, raising the specter of a long legal battle over her extradition.
After nearly 12 hours of deliberations, the court reinstated the guilty verdict first handed down against Knox and Raffaele Sollecito in 2009.
The verdict had been overturned in 2011 and the pair freed from prison, but Italy's supreme court vacated that decision and sent the case back for a third trial in Florence.
Sollecito, whose lawyers said they would appeal the verdict, was sentenced to 25 years. Reached by telephone, Knox's father, Curt Knox, said he had no comment.
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Sollecito's lawyers said they were stunned and would take their appeal to Italy's top court. "There isn't a shred of proof," said attorney Luca Maori said.
Presiding Judge Alessando Nencini ordered the 29-year-old Sollecito's passport revoked but made no requests for Knox's movements to be limited, saying she was "justifiably abroad."
Knox's defense team gave its last round of rebuttals earlier in the day, ending four months of arguments in Knox's and Sollecito's third trial for the 2007 murder of her British roommate, Meredith Kercher, in the Italian university town of Perugia.
"It is not possible to convict a person because it is probable that she is guilty," Dalla Vedova said. "The penal code does not foresee probability. It foresees certainty."
Dalla Vedova evoked Dante, noting that the Florentine writer reserved the lower circle of hell for those who betrayed trust, as he asserted that police had done to Knox when they held her overnight for questioning without legal representation and without advising her that she was a suspect.
She told Italian state TV in an interview earlier this month that she would wait for the verdict at her mother's house "with my heart in my throat."
Knox's absence didn't formally hurt her case since she was freed by a court and defendants in Italy are not required to appear at their trials.
However, Nencini reacted sternly to her emailed statement, noting that defendants have a right to be heard if they appear in person.