"We demand that an inquiry into the case be done by an independent investigative body headed by a High Court judge and not by state police or an agency influenced by the government," Pilot told reporters in Dausa.
He said Congress leaders have no faith in BJP government in the state which he alleged in indulging in "politics of vendetta".
The Rajasthan police had yesterday filed an FIR naming former Chief Minister Ashok Gehlot, Pilot and several others in an ambulance scam.
Rajasthan police filed the FIR over allegations of financial irregularities to the tune of Rs 2.56 crore in operating '108' ambulance service during the previous Congress regime.
Former state health minister A A Khan, Karti Chidambaram, son of former Union Minister P Chidambaram and Ravi Krishna, son of former Union Minister Vayalar Ravi, Sweta Mangal, then CEO of Ziqitza Healthcare, Shaffi Mather, former Economic Advisor to Kerala Chief Minister Oommen Chandy and the Director of NRHM also figured in the FIR.