Joining the protesters at Azad Maidan in South Mumbai, the Sena chief attacked Chief Minister Devendra Fadnavis and said the state government will be "cursed by mothers".
Anganwadi workers have been on an indefinite strike across the state since September 12 demanding a raise in their salaries as per their service seniority.
The government on September 23 decided to hike the payment of anganwadi workers to Rs 6,500 per month, which they had termed as "minuscule".
Appealing the workers to stand their ground, Thackeray, whose party shares an uneasy relationship with the BJP in the state government, said, "You have the power to make the government bow before you".
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"...Our hearts are still alive. We cannot work like corpses. The chief minister should keep aside his ego and give justice to the workers. If you want to do whatever you like to in the name of democracy, we will not let that happen," he said.
Meanwhile, the workers today threatened to enter the state Secretariat if their demands are not met within eight days.
Nearly two lakh Anganwadi workers working in 1.10 lakh Anganwadis in the state cater to nutritional needs of children under the age of six and adolescent girls who are malnourished or anaemic.
The beneficiaries of these schemes implemented by Anganwadi workers are provided two freshly cooked meals.
Sena has of late intensified its attack on its senior alliance partner, the BJP, and has often taken potshots at Prime Minister Narendra Modi.
The Uddhav Thackeray-led party had recently indicated that it was close to pull out of the coalition government in the state. However, the BJP has ruled out any threat to the survival of the government.
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