The 40-year-old Oscar-winning actress, who will reprise her role as the Tigress, will have her children lending their voices to some of the other pandas featured in the film, reported E! online.
"They have some lines in the film," Jolie's representative said.
Though this will be the first big screen performance for Knox, 7, with his mom, the rest of the kids have worked on the set with Jolie and dad Brad Pitt.
Shiloh, 9, and Maddox both worked on their father's "The Curious Case of Benjamin Button" and "World War Z".