Turkey last month launched military operation dubbed "Olive Branch" supporting Syrian rebels with ground troops and air strikes against the Kurdish Peoples Protection Units (YPG) militia viewed as "terrorists" by Ankara.
The Turkish source was reacting to claims by the head of Afrin hospital in northern Syria that six men were treated late Friday after shelling during the offencive and had symptoms that were in line with exposure to toxic agents.
Observatory chief Rami Abdel Rahman told AFP he could not confirm whether toxic gases were used.
But the Turkish source said Ankara took the "utmost care" regarding civilian safety and that the claims Turkey was responsible for an alleged gas attack were "baseless".
"Turkey never used chemical weapons," the source added. "(These are) lies... this is black propaganda."
Turkey says the YPG is linked to the outlawed Kurdistan Workers Party (PKK), which has been waging an insurgency against the Turkish state since 1984.
But the YPG has been working closely with Washington to oust the Islamic State extremist group from Syria, which has strained relations between the two NATO allies.
The Observatory says at least 78 civilians have died during Turkeys operation but Ankara repeatedly insists it is taking all the necessary measures to protect civilians.
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