The clear Oral B-40 toothbrush was used by command module pilot Jack Swigert during the 1970 Apollo 13 mission and sold for USD 11,974, said the Nate D Sanders auction house, which did not reveal the buyer.
Swigert was played by actor Kevin Bacon in the 1995 movie, "Apollo 13," which also starred Tom Hanks.
The mission was meant to be the seventh manned journey to land on the lunar surface, but the touchdown was aborted after an oxygen tank exploded 55 hours into the trip.
Swigert was one of 24 American astronauts to fly around the Moon as part of the Apollo program, but was not among the dozen who walked on the surface.
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He died in 1982 of bone cancer at age 51.
The auction of personal items from his estate fetched a total of USD 224,940, the auction house said.
The most expensive item was a silver Robbins medal that Swigert was given for the Apollo 17 flight, the final lunar landing of the program, which sold for USD 56,250.
An Apollo-13 flown flag sold for USD 18,291, while an earpiece Swigert wore in space sold for more than USD 6,000.
Last week, a joystick controller from the fourth US manned lunar landing, Apollo 15, sold at a Massachusetts auction for USD 610,063.
That was believed to be the most ever paid at auction for a piece from a NASA spacecraft, experts said.