However, Shanghai surged after data showed Chinese inflation held above two percent for a second-straight month in March, indicating some stability in the economic powerhouse.
The broad regional losses follow last week's sell-off as traders become increasingly fearful central banks are running out of ideas to ramp up global growth, with the International Monetary Fund expected to lower its forecasts this week.
In Tokyo the benchmark Nikkei ended 0.4 per cent lower as exporters took another hit from the yen's strength. The unit, which is considered a safe bet in times of uncertainty, has climbed about five per cent against the greenback this month alone.
On Friday top Japanese government spokesman Yoshihide Suga tried to talk down the yen, saying its rise was "one- sided", while Finance Minister Taro Aso similarly said rapid moves in the currency were undesirable.
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His warning had an initial impact as the yen temporarily weakened but it later resumed its rally.
Today Suga added that the government was "closely watching with vigilance, and is ready to take necessary measures depending on situations".
"Players are turning pessimistic as the stock market is weak, and the yen's strengthening will likely continue for a while," he said.
The generally gloomy mood was evident across Asian markets, with Sydney and Seoul each 0.1 per cent lower, while Wellington, Jakarta and Singapore were also down.
In early European exchanges London and Frankfurt each dropped 0.3 per cent and Paris shed 0.2 per cent.
But Shanghai closed up 1.6 per cent - and helping Hong Kong to a 0.4 per cent rise - as dealers welcomed figures showing inflation held steady last month.