Assistant Police Inspector Sachin Suryavanshi appeared before the seven-member panel headed by senior-most member of the state assembly Ganpatrao Deshmukh of Peasants and Workers Party.
Suryavanshi's deposition lasted for about 90 minutes, assembly officials said.
However, what transpired during the deposition was not immediately known.
MLAs Kshitij Thakur of Bahujan Vikas Agadhi and Ram Kadam (MNS), who were arrested in connection with the assault on the officer, and security staff of the House will also appear before the committee, the officials said.
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Buying peace with legislators who disrupted the proceedings of the two Houses of legislature, Home Minister R R Patil on March 25 had suspended Suryavanshi for "misbehaving" with Thakur and ordered constitution of a panel to go into the entire episode.
Suryavanshi, attached to the Worli police station, was thrashed by a group of MLAs outside the visitors gallery at the state's legislature complex on March 19, a day after he had stopped Thakur's vehicle on Bandra-Worli Sea Link for speeding and slapped a fine.