The storyboard from the 18th comic in the popular French series will carry a special dedication by co-creator Albert Uderzo while auction house Christie's said it would waive its commission for the charity sale.
The artwork, which was sold at an auction yesterday dedicated to comic books, had been valued at between 150,000 and 200,000 euros (USD 157,000- USD 209,000). There was no immediate information about the buyer.
Two days after the shootings, Uderzo came out of retirement to pen cartoons in tribute to the victims, who included some of the country's most celebrated cartoonists.
"Moi aussi je suis un Charlie" ("I'm Charlie too") says Asterix in one of the cartoons, echoing the "Je Suis Charlie" slogan adopted by people around the world protesting at the attack on freedom of expression. The drawing also showed the Gallic cartoon hero punching an adversary high into the air.
"Charlie Hebdo and Asterix are very different, of course. I am not going to change my stripes," 87-year-old Uderzo told Europe 1 radio at the time.
"I simply wanted to show my friendship for these cartoonists who have paid with their lives.