The two vehicles were on their way to a canal for a ceremony related to Bhagwat puja which was being organised in Pura village, Additional Superintendent of Police R P Gupta said.
The speeding truck rammed into one of the tractor-trolley near Harchandpur village on Mainpuri road. Due to the high impact of this collision, the damaged trolley climbed on the trolley in front of it, the ASP said.
Five of the deceased were children and 12 were women, he said.
Nine of them are in serious condition and four are stated to be critical, he said.
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Expressing grief over the incident, Uttar Pradesh Chief Minister Akhilesh Yadav announced financial assistance of rupees two lakh each to kin of those killed in the accident and Rs 50,000 each to injured.
He also directed the officials to provide free treatment to the injured.
Meanwhile, local residents staged an agitation by blocking a road near Harchandpur area and started stone-pelting on the police, administrative officers and the vehicles passing by.