Images of the fiery accident yesterday on a busy bridge showed extensive damage to the left side of the bus, part of which was completely sheared off by the force of the crash.
Melissa Mixon, spokeswoman for North Seattle College, told AFP that 45 students and staff with the school's international program were on board the bus.
She could not immediately provide information on the nationality or age of the victims.
College president Warren Brown told reporters the students were going to visit Safeco Field, a sports stadium, as part of their orientation program.
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Seattle Mayor Ed Murray said authorities were assisting families of the victims who were flying into Seattle from several countries.
"The accident occurred when a charter bus traveling with the students collided with a 'Ride the Ducks' tour vehicle," Mixon said.
The college hosts some 1,000 international students from 50 countries, including China, Korea and Japan, according to its website.
The school year is scheduled to begin on Monday.