The latest incident in which the Lankan Navy attacked some fishermen from Rameswaram fishing near Katchatheevu "has deeply saddened us", Karunanidhi said in a statement.
Stating that the incident had occurred the same day when Prime Minister Narendra Modi was meeting Tamils in the island nation, he said more than 30 Sri Lankan Navy personnel came in five boats, attacked the fishermen and damaged fishing equipment.
"India should urge the Sri Lankan President to advice the Navy personnel in order to reach a solution in this issue," he said.