The Australia women's team, which had thrashed India A by 321 runs in the first warm up game on Tuesday, continued its winning streak.
After electing to bat, the hosts were bundled out for a partly 170 as the Australian bowlers put up a dominant performance.
Led by Amanda Jade Wellington (3-30), the visitors ran through the Indian batting line up. Jade was superbly aided by Ashleigh Gardner (2-32).
For the hosts, skipper Anuja Patil top scored with 49 at the Mumbai Cricket Association Bandra Kurla complex facility.
Now the Australia Women engage in a three match ODI series versus India starting from March 12 at Vadodara.
Brief scores: India A 170 all out (Anuja Patil 49, Amanda Jade Wellington 3-30) lost to Australia Women 171/3 (Meg Lanning 63) by seven wickets.