Saeed Noori had been charged with 18 counts of attempted murder and one count of conduct endangering life alleging he drove an SUV into pedestrians on December 21 on a sidewalk in downtown Melbourne, Australia's second-largest city.
An attempted murder charge was upgraded in the Melbourne Magistrates Court to murder because an 83-year-old man died after eight days in hospital.
Another three victims remain in hospital. Murder carries a potential maximum sentence of life imprisonment, while attempted murder carries a 25-year sentence.
It was the second alleged use of a car to assault pedestrians in downtown Melbourne in 2017. In January, six people were killed and more than 30 were injured when a car was driven up a pedestrian mall.
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The defendant in that case pleaded not guilty to murder and other charges last month, and his defence lawyers have also questioned his mental health and fitness to enter a plea.
In October, a man drove a truck down a New York City bicycle path, killing eight people. The driver is accused of providing material support to the Islamic State group among other charges, including murder.
In August, 13 people were killed and more than 100 were wounded when a vehicle rammed into pedestrians in Barcelona, Spain.
Two attacks in London last year were allegedly linked to Islamic extremists and another was seen as a reprisal attack outside a mosque, killing 13 people altogether.