The court passed the order, nearly three hours after Tazreen Fashions Managing Director Delwar Hossain and and his wife Mahmuda Akter, who is also the chairperson, surrendered before it seeking bail in the case.
Their surrender came as a lower court declared them fugitive and issued an arrest warrant in December.
Delwar and Mahmuda surrendered before the court of Senior Judicial Magistrate Tajul Islam this morning, the Daily Star reported.
On December 22 last year, 13 people, including the owners of a garment manufacturing unit, were charged with culpable homicide for the death of 112 people in the blaze.
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At least 112 workers, mostly women, were killed and scores wounded in the country's worst industrial fire at Tazreen Fashions in Ashulia on the outskirts of the capital on November 24 in 2012.
The fire was the country's deadliest ever industrial accident till the Rana Plaza garment factory complex collapsed just months later, killing more than 1100 people.
Bangladesh's garment industry is worth over USD 20 billion, second only to China, but is often in the international spotlight because of poor safety conditions.
Factory owners are rarely charged over such accidents in the sector, which employs over 3.6 million workers, mostly women, and accounts for 77 per cent of the country's exports.
Bangladesh has pledged to clean up the industry and more than 100 top Western retailers have agreed on new safety measures to allow greater scrutiny of their operations.