A division bench of Justices Mohinder Pal and R D Kothari ordered that Bajrangi's application be "dismissed as withdrawn".
Bajrangi had moved the HC through his advocate Pravin Gondaliya seeking regular bail on medical grounds for treating eye ailment and also sought suspension of the sentence awarded to him by a special trial court.
However, the HC observed the plea should be rejected as bail cannot be granted only on medical grounds because any prisoner can be provided with treatment as per the jail manual and existing laws.
Bajrangi is out of jail since April 24 after a division bench of the HC comprising Justices Ravi Tripathi and R D Kothari granted him bail for 90 days for treatment of his eyes.
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The VHP leader had been awarded life imprisonment in August 2012 along with then BJP MLA Maya Kodnani and 29 others in connection with the Naroda Patiya riot case in which 97 people were killed in the communal violence.
The massacre had taken place a day after a coach of Sabarmati Express was burnt down by some assailants near Godhra railway station on February 27, 2002 which caused death of 54 'karsevaks'.
Bajrangi is also one of the 83 accused in the Naroda Gam riot case in which 11 persons of the minority community were killed on February 28, 2002.