Private bus operators suspended their services across the state as bandh supporters burnt tyres and logs on the state and national highways to enforce the bandh.
Bandh supporters blocked railway tracks in Patna, Sitamarhi, Samastipur and Saharsa. Several trains were running behind their schedule, sources said.
They also blocked national highways at different places in Aurangabad, Sitamarhi, Motihari, Muzaffarpur and Purnia.
Yadav was taken into custody when he took out procession in Saharsa town to enforce the call while Hussain, was detained in Bhagalpur town, officials said.
Talking to reporters, Yadav demanded that Prime Minister Manmohan Singh resign immediately for "failure" of his government to control the spiralling prices of petroleum products and essential commodities.
Several other senior JD(U) and BJP leaders like MP Arjun Rai were also detained while trying to enforce the bandh call at different places in the state.
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Major shops, business establishments and schools remained closed in Patna, Saharsa, Motihari, Bettiah, Madhepura, Muzaffarpur, Bhagalpur, Darbhanga, Sitamarhi and Madhubani districts.
Vehicular traffic too plied in depleted numbers in these towns. (MORE)