Luis Enrique's side were taken apart in the first leg in Turin, losing 3-0 to a Juve team inspired by Paulo Dybala, who scored twice.
In the last 16 they became the first team ever to overturn a 4-0 deficit when they beat Paris Saint-Germain 6-1 in the return at the Camp Nou, so fans are hoping for a similar "remuntada" this time.
"We are going to take as many risks as we need to the point that we could go out with eight strikers, as we have nothing to lose," said Luis Enrique after Saturday's 3-2 defeat of Real Sociedad.
But he added: "The thing is that we are used to always eating beluga caviar. That can't go on forever."
More From This Section
Barca welcome back midfielder Sergio Busquets from suspension, while Juve are hopeful Dybala will be ready after he came off in the 2-0 weekend win at Pescara with a sprained ankle.
In contrast to their fierce rivals, Real Madrid are very much on course for a semi-final berth after their 2-1 win away to Bayern Munich last week.
Bayern coach Carlo Ancelotti has fitness concerns with leading scorer Robert Lewandowski hopeful of returning from a shoulder injury.
Defenders Mats Hummels and Jerome Boateng are also fighting to be fit, while Javi Martinez is suspended.
Gareth Bale is struggling to shake off a calf problem for Real, who have lost just twice in 12 past home meetings with Bayern.
- Dortmund test of character -
==============================
Dortmund went on to lose 3-2 to the French league leaders but morale was lifted as they got back to winning ways in the Bundesliga at the weekend, beating Eintracht Frankfurt 3-1.
After that game, the Dortmund players paid tribute to defender Marc Bartra by holding his number five shirt aloft as they celebrated in front of their fans.
Spanish international Bartra had earlier been released from hospital after having surgery on a broken wrist suffered in the attack.
Monaco, looking to become the first French side in the Champions League semi-finals since Lyon seven years ago, are missing the banned Fabinho as well as Djibril Sidibe, who is recovering from appendicitis.
Leicester City have defensive concerns as they look to turn around a 1-0 deficit when they host last season's runners-up Atletico Madrid.