Beef requires 28 times more land than the average total needed to produce either dairy, eggs, poultry or pork, said the research in the Proceedings of the National Academy of Sciences.
Raising beef also requires 11 times more irrigation water than other proteins, according to researchers at Bard College in New York, Yale University in Connecticut and the Weizmann Institute of Science in Rehovot, Israel.
Beef spews far more pollution into the environment, producing five times as many greenhouse gas emissions and six times the reactive nitrogen from fertiliser compared to the other proteins, the study found.
Beef also makes up about seven per cent of all consumed calories in the US diet, it said.
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To "most effectively" cut back on these environmental costs, the authors recommended "minimising beef consumption."
Raising livestock for food is a practice that contributes to one fifth of global greenhouse gas emissions, and also pollutes water and interferes with biodiversity, according to the study authors.
The study was based on a decade's worth of data on land, irrigation water, and fertiliser from the US Department of Agriculture, the Department of the Interior, and the Department of Energy.
About every 10 calories fed to poultry or pork accounted for one calorie consumed by humans. This ratio was nearly four times higher for beef.
Poultry, pork, eggs and dairy all added up to similar costs across the board, while beef was consistently the outlier.
They did not include fish in their study due to lack of data on feed use and the relatively small portion of calories (0.5 per cent) it makes up in the average American diet.
Representatives of the US beef industry questioned the methodology of the study, and said environmental improvements have been made in recent years.