The 34-year-old megastar's crew joined her in giving millions of viewers a black-power salute as Beyonce belted out her new politically charged power anthem, "Formation."
"OK, ladies, let's get in formation!" she sang, tweaking the 50th anniversary of the Super Bowl with a nod to the 50th anniversary of the 1966 founding of the Black Panther Party.
At one point, her dancers formed an X on the field, an apparent reference to Malcolm X, the black rights campaigner.
Other than that, Beyonce danced side by side with Bruno Mars and, in a grand finale, with Coldplay's Chris Martin as the audience spelled out "Believe in Love" with rainbow-coloured placards.
For a very brief moment during her energetic performance, the star nearly stumbled, but she recovered in no time.
The Pepsi Super Bowl 50 halftime took place at Levi's Stadium in the San Francisco Bay Area.