Protestors stopped trains in Mathura, Agra, Varanasi, Allahabad and Lucknow as part of their stir while BJP workers and traders blocked the Agra-Gwalior Highway by burning tyres.
Major markets remained closed at a number of places in UP, including the state capital, official sources said here.
Raising slogans against FDI in retail, a group of workers held a demonstration outside the Walmart store at Sultanpur road in Lucknow.
Several prominent schools remained closed as a precautionary measure in the city.
In Lucknow, BJP's former national president Rajnath Singh led the protest, whereas national vice-president Mukhtar Abbas Naqvi was leading the protest in Kanpur.
Reports of similar protests also poured in from other districts including Allahabad where activists of the youth wing of the ruling SP stopped the Bareilly-bound Triveni Express at 5.30 AM on Niranjan railway bridge.
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They protesters assembled in large numbers along the railway tracks and also burnt the effigies of the Prime Minister and Congress President Sonia Gandhi.
The agitation delayed the onward journey of the train by an hour.
SP has directed its workers not to take law in their hands and hold peaceful protest, SP spokesman Rajendra Chowhdury said.