The partnership will commence with the Monte Carlo Masters, beginning April 14 and probably end when Bhupathi hangs up his boots at the end of the season.
Bhupathi and Bopanna, country's top doubles players ranked 10th and 12th respectively, had teamed up in the 2012 season aiming for an Olympic medal but split for the year 2013 as they looked for young partners.
The decision was basically to help 33-year-old Bopanna get better results since Bhupathi's reflexes were slowing due to age factor. He is 38.
Bhupathi had played with Canada's Daniel Nestor and Michael Llodra while Bopanna played with American Rajeev Ram and Briton Colin Fleming.
"We had a really good run last year, and had found a rhythm in our game while having a great time playing together. We have a refined understanding of each other's games, and gel well on and off the court; all strong characteristics that I know will translate into a successful partnership for us on the tour," Bhupathi said.