Biden's comments came at a memorial to the five servicemen killed in a shooting rampage by Kuwait-born Muhammad Youssef Abdulazeez.
"These perverse ideologues, warped theocrats, they may be able to inspire a single lone wolf to commit a savage act, but they can never, never threaten who we are," Biden said. "When this perverted jihadist struck, everyone responded."
The vice president's strong words contrast with the official comments of investigators who have not yet been able to determine a motive behind the attack.
Speaking before Biden, Secretary of Defense Ash Carter said officials may never know "what combination of disturbed mind, violent extremism and hateful ideology" was behind the shooting, but vowed that the United States would present a strong response.
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"The few who threaten or incite harm to Americans - violent extremists or terrorists, wherever they are - will surely, very surely, no matter how long it takes, come to feel the long arm and the hard fist of justice," Carter said.
Abdulazeez began the July 16 rampage by opening fire on a military recruiting center before driving his rented Mustang about 7 miles across town to a reserve center, crashing through the gates and killing the five servicemen. Abdulazeez died in a shootout with police.
Friends and neighbors recalled Abdulazeez as a happy, polite young man. But a picture has also emerged showing a darker side, with Abdulazeez's family saying he struggled with depression from his early teens, abused drugs, couldn't keep a job and was considering bankruptcy.